Immunofluorescence detection of WT1 in HS207, HS360 and HS401 cells cultured in suspension.
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Nuclear expression of WT1 in cells was observed in all hES cell lines after spontaneous differentiation (A, C, and E) as well as after BMP7-stimulation (B, D and F) in cells mainly located on the edge of the spheres. Nuclear expression of WT1 was observed in Sertoli cells present in adult human testis (G; arrow heads). Negative controls exhibited no specific WT1 staining in either the nucleus or cytoplasm (H and small inserts in a-f). Red: WT1 staining. Blue: DAPI staining marking the nucleus. Scale bars: 100 μm in A to H, and 50 μm in the small inserts.