Immunofluorescence detection of SOX9 in HS207, HS360 and HS401 cells cultured in suspension.
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Cytoplasmic expression of SOX9 in cells on the edges of the cell spheres was observed in all hES cell lines after BMP7-stimulation (B, D and F) as well as after spontaneous differentiation (A, C and E). Nuclear expression of SOX9 was observed in Sertoli cells present in adult human testis (G; arrow heads). Negative controls exhibited no specific SOX9 staining in either the nucleus or cytoplasm (H and small inserts in A to F). Green: SOX9 staining. Blue: DAPI staining marking the nucleus. Scale bars: 100 μm in A to H, and 50 μm in the small inserts.