Immunofluorescence confirms Cerulean, mCherry, and YFP transgenes are each expressed in a distinct cell type.
Combined confocal fluorescence (Cerulean, light blue, mCherry, red, YFP, yellow), immunofluorescence (primary antibodies detected with far red secondary antibodies, white), and two-photon (DAPI, nuclei, dark blue) microscopy from cortex, just above corpus callosum, on adult mice. A) Colocalization of Aldh1L1::Cerulean-Myc (light blue), with all cells (red arrows) immunofluorescent for Aldh1L1 protein (white). B) Colocalization of Snap25::mCherry (red), with all cells (yellow arrows) immunofluorescent for NeuN nuclear protein (white). C) Colocalization of Mobp::YFP-HA(light blue), with all cells (red arrows) immunofluorescent for Cnp1 protein (white). D) Lack of colocalization of any fluorophore with Ng2+ (white) cells (yellow arrow).