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Immunofluorescence confirms Cerulean, mCherry, and YFP transgenes are each expressed in a distinct cell type.

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posted on 12.07.2012, 00:19 by Joseph D. Dougherty, Juliet Zhang, Huifen Feng, Shiaoching Gong, Nathaniel Heintz

Combined confocal fluorescence (Cerulean, light blue, mCherry, red, YFP, yellow), immunofluorescence (primary antibodies detected with far red secondary antibodies, white), and two-photon (DAPI, nuclei, dark blue) microscopy from cortex, just above corpus callosum, on adult mice. A) Colocalization of Aldh1L1::Cerulean-Myc (light blue), with all cells (red arrows) immunofluorescent for Aldh1L1 protein (white). B) Colocalization of Snap25::mCherry (red), with all cells (yellow arrows) immunofluorescent for NeuN nuclear protein (white). C) Colocalization of Mobp::YFP-HA(light blue), with all cells (red arrows) immunofluorescent for Cnp1 protein (white). D) Lack of colocalization of any fluorophore with Ng2+ (white) cells (yellow arrow).

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