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Immunoelectron microscopic localization of perilipin in briefly AIM-stimulated human preadipocytes.

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posted on 28.02.2014 by Hans Heid, Steffen Rickelt, Ralf Zimbelmann, Stefanie Winter, Heiderose Schumacher, Yvette Dörflinger, Caecilia Kuhn, Werner W. Franke

(a) Shown are groups of LDs positive for perilipin by nanogold-label and silver enhancement. Localization was with mab Peri112.17. The LDs are seen closely associated and anchored with IF bundles. (b) Enlarged perilipin labeling with two small LDs (approximately 300–400 nm in diameter) approaching a big LD (approximately 2.0–2.5 µm in diameter) for combining and coalescence. Bundles of intermediate-sized filaments are associated to grains of the immunolabeled perilipin (arrows). Bars: 0.50 µm.