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Immunodetection of Fox2p, Icl1p and cytochrome c by Western-blot in the C. lusitaniae fox2Δ and wild-type strains.

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posted on 08.12.2014 by Frédéric Gabriel, Isabelle Accoceberry, Jean-Jacques Bessoule, Bénédicte Salin, Marine Lucas-Guérin, Stephen Manon, Karine Dementhon, Thierry Noël

(A) Thirty µg of proteins of the crude extracts and 10 µg of proteins of the peroxisomal and mitochondrial fractions were separated by SDS-PAGE. (B) Signal integration expressed in relative intensity using Quantity One software. Relative amounts of Fox2p are represented with black bars, Icl1p with grey bars, and Cytc with white bars. Error bars represent SE. Cytc: cytochrome c, WT: wild type, CE: crude extract, P: peroxisomal fraction, M: mitochondrial fraction.