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Immunocytochemistry of LARGE transgenic mouse brain.

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posted on 20.02.2013, 21:40 by Martin Brockington, Silvia Torelli, Paul S. Sharp, Ke Liu, Sebahattin Cirak, Susan C. Brown, Dominic J. Wells, Francesco Muntoni

Cryosections of brains from LARGE transgenic (line 68) (A–C,G–L) and wild type littermates (D–F) at 2 months of age. Sections were stained using antibodies: V5 (A,D,G,J); α-DG (IIH6) (B,E and H); GM130, a Golgi marker, (K). Nuclei were stained with Hoechst 33258 (blue). C,F,I and L are merged images. Cells expressing different levels of V5 (LARGE) were seen in the hippocampal pyramidal layer (A) while IIH6 α-DG stained only vessels (B). In the cerebellum, immunolabelling of V5 was detected in Purkinje cells (G, white arrow). In these cells IIH6 α-DG was seen around the cell soma (H, arrow), in a similar level to wild type Purkinje cells (E, arrow). Colocalisation of V5 and GM130 in neuronal cells of the cerebral cortex (L) shows that the transgene was localised to the Golgi.

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