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Immunocytochemical identification of cultured astrocytes dissociated from the CA1 region of the hippocampus.

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posted on 27.06.2012, 02:41 by Olena Butenko, David Dzamba, Jana Benesova, Pavel Honsa, Valentina Benfenati, Vendula Rusnakova, Stefano Ferroni, Miroslava Anderova

(A) The distinct morphology of astrocytes isolated from sham-operated rats or from the ischemic hippocampal CA1 region: flat astrocytes (top) and non-flat astrocytes (bottom) cultured for 4–5 days. Both types of astroglial cells expressed GFAP and GLAST and were also positive for TRPV4. (B) The incidence of flat and non-flat astrocytes in controls (CTRL) and those isolated from the CA1 hippocampal region 1H and 7D after H/I (n =  number of coverslips). The values are presented as mean ± S.E.M. Statistical significance was calculated using one-way ANOVA. *p<0.05, significant; ***p<0.001, extremely significant.