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Immunoblotting analysis of ILK1, pAKT and EGFR in PF4-DLR treated glioblastomas.

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posted on 29.10.2010, 00:35 by Chiara Verpelli, Giulio Bertani, Valentina Cea, Monica Patti, Andreas Bikfalvi, Lorenzo Bello, Carlo Sala

Tissue lysates of the treated (D10 and D20) and untreated (c10 and c20) tumors were prepared as described in Materials and Methods, and the proteins were resolved on 10–12% PAGE gels. A) The proteins were blotted onto a nitrocellulose membrane and detected with antibodies raised against the individual proteins indicated on the right side of each panel. B) Histograms of the mean band intensity (± SEM) of each protein. *p<0.05, t test.

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