Immunoblotting analysis of ILK1, pAKT and EGFR in PF4-DLR treated glioblastomas.
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Tissue lysates of the treated (D10 and D20) and untreated (c10 and c20) tumors were prepared as described in Materials and Methods, and the proteins were resolved on 10–12% PAGE gels. A) The proteins were blotted onto a nitrocellulose membrane and detected with antibodies raised against the individual proteins indicated on the right side of each panel. B) Histograms of the mean band intensity (± SEM) of each protein. *p<0.05, t test.