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Immunoblot assay showing lipid peroxidation mediated membrane damage.

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posted on 13.09.2013 by Sumanti Gupta, Anirban Bhar, Moniya Chatterjee, Sampa Das

Lane1; positive control showing MDA-BSA bands at 82kDa and 182kDa respectively. Lane 2 and 3; total protein profile of uninduced roots of JG62 and WR315 respectively. Lane 4, 6, 8 and 10; total protein profile of infected roots of JG62 at 2dpi, 4dpi, 7dpi and 12dpi respectively. Lane 5, 7, 9 and 11; total protein profile of infected roots of WR315 at 2dpi, 4dpi, 7dpi and 12dpi. Each lane contains 10 µg of total protein uniformly.

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