Immunoblot analyses of endogenous HPV16 E6, upon intracellular expression of E6-targeting peptides.
Expression of either hrGFP-linked peptides pep11**m or pep11’m (both E6-binding defective), or pep11**, pep11’ or E6APpep (all E6-binding competent) in HPV-16 positive MRI-H186 cells. Soluble and insoluble protein fractions are indicated. Loading of protein extracts was normalized for equal transfection efficiencies, as determined by the activities of a co-transfected β-galactosidase expression vector. Expression levels of endogenous E6AP, of p53, and of individual peptide-hrGFP fusion proteins are indicated. α-Tub, α-tubulin; s. exp., short exposure; l. exp., long exposure.