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Immunization schedule and expression of recombinant truncated fragment proteins.

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posted on 2014-04-15, 02:59 authored by Qian-Fei Zuo, Chang-Zhi Cai, Hong-Lei Ding, Yi Wu, Liu-Yang Yang, Qiang Feng, Hui-Jie Yang, Zhen-Bo Wei, Hao Zeng, Quan-Ming Zou

(A) Diagrams showing the experimental design of the immunization schedule for the measurement of antibodies, the survival rates up to 14 days after bacterial challenge and the bacterial budens on days 1 and 3 after bacterial infection. (B) Structural organization of the fibronectin-binding protein, FnBPA from S. aureus strain MRSA252 and schematic diagram illustrating the primary structure of the FnBPA1-965, F130-500(F1) and F2501-941(F2). (C) Recombinant GST-tagged F1 and F2 were purified by affinity chromatography and analyzed by SDS-PAGE.