Immune reconstitution in recipient fah-/- mice after syngeneic BMT.
(A) Donor-derived EGFP+ PBMC measurements from BMT mice at the indicated time points. (B) PBMC subset (NK, B, CD4+, and CD8+ T) measurements from BMT mice at the indicated times. PBMC from EGFP-Tg mice was set as a positive control. (mean ± SEM, n = 9). (C) Thymus, spleen, and liver CD4+ or CD8+ T lymphocyte detection in BMT mice (rebuild 9w) or EGFP-Tg donor mice by FACS. (D) Spleen and inguinal LN histology of serial sections of BMT mice (rebuild 9w). From L-R, H&E stain, EGFP, CD3, and CD19 immunohistochemistry. Brown staining indicates positive cells. (Original magnification 100×). (E) Serum anti-HBsAg levels in EGFP-BMT and EGFP-Tg mice after twice HBV vaccine immunizations.