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Imaging-based quantification of leukemia initiating cell frequencies. a

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posted on 31.12.2012 by Nadia Terziyska, Catarina Castro Alves, Volker Groiss, Katja Schneider, Katarina Farkasova, Manfred Ogris, Ernst Wagner, Harald Ehrhardt, Renier J. Brentjens, Udo zur Stadt, Martin Horstmann, Leticia Quintanilla-Martinez, Irmela Jeremias

Imaging visualizes dependence of leukemic growth on both time and cell numbers; experiment shown in Figure 2b was followed up over time in all groups injected with the different cell numbers; depicted is the quantification of imaging as mean of each group +/− SEM; b Imaging enables convenient determination of CSC frequencies; ALL-54 cells were freshly isolated from a mouse spleen, seeded at 106 cells/ml and stimulated in vitro with PBS or TRAIL (1 µg/ml). After 48 hours, cells were serially diluted based on the cell concentration seeded at the beginning of the experiment and injected into groups of 2–3 mice. After 8 weeks, mice were imaged and analyzed for leukemic engraftment (defined using signals of legs only, identically as in Figure 3D; engraftment is indicated with a star); frequency of leukemia initiating cells was calculated out of engraftment rates using Poisson statistics. In all mice, mid-abdominal signals are unspecific.

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