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Identification of infiltrating cells by immunocytochemistry.

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posted on 21.02.2013, 12:24 authored by Simona Ozden, Michel Huerre, Jean-Pierre Riviere, Lark L. Coffey, Philippe V. Afonso, Vincent Mouly, Jean de Monredon, Jean-Christophe Roger, Mohamed El Amrani, Jean-Luc Yvin, Marie-Christine Jaffar, Marie-Pascale Frenkiel, Marion Sourisseau, Olivier Schwartz, Gillian Butler-Browne, Philippe Desprès, Antoine Gessain, Pierre-Emmanuel Ceccaldi

Muscle sections from patient#1 (A to E)) or patient#2 (F to J) werre processed as described in material and methods for immunocytochemistry (immunoperoxydase detection) for the following antigens: CD56 (mouse monoclonal antibody; Dako; A,E); CD8 (mouse monoclonal antibody; Dako; B,G); CD20 (mouse monoclonal antibody; Dako; C,H); CD3 (Neomarker; D,I); CD68 (mouse monoclonal antibody; Dako; E,J). In patient#1 (A to E), the few infiltrating cells were mostly detected as immunoreactive for CD68 and CD3. In patient#2, the massive infiltrates were mainly composed of CD68 and CD3 immunoreactive cells. Counterstain: henatoxylin-eosin. Immunoreactive cells (red-brown color) are indicated by arrows, except in I and J (too numerous). Magnification: ×170.


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