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Identification of endolysin.

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posted on 2013-11-13, 02:54 authored by Wenhui Zhang, Zhiqiang Mi, Xiuyun Yin, Hang Fan, Xiaoping An, Zhiyi Zhang, Jiankui Chen, Yigang Tong

A. Amino acid sequence of IME-EF1 endolysin was aligned and phylogenized with the submitted, confirmed, or putative endolysin from six strains of E. faecalis bacteriophage in GenBank. B. SDS-PAGE and Western blot were used to identify the expression of endolysin in E. coli M15. Lanes 1 and 4 indicate the negative control with the cells transformed with the empty vector pColdI. Lanes 2 and 5 show the cells transformed with recombinant pColdI containing IME-EF1 endolysin gene, whereas Lane 3 was pre-stained with the protein marker. Lanes 1 to 3 were stained with Coomassie brilliant blue, whereas Lanes 4 and 5 were transferred to the PVDF membrane and blotted with anti-His antibody. The black arrow indicates the expressed endolysin.


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