Identification of Garcinol as a natural product biologically active on HSCs/PCs. A.
Experimental design for the screening of natural compounds acting on HSCs/PCs. B. Chemical structure of Garcinol and its derivatives. C. Garcinol expands CD34+CD38– cell numbers. hCB CD34+ cells were cultured in StemSpan SFEM medium with Garcinol or the same volume of DMSO (blank) in the presence of 20 ng/mL of hrTPO and 100 ng/mL of hrSCF. At day 7 of culture, the cells were analyzed by FACS for CD34 and CD38 expression (left panel). The numbers of CD34+CD38– cells relative to the blank are indicated [mean±standard error of the mean (SEM), n = 3]. *p<0.05.