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IS-induced PRR activation is involved in cell proliferation and tissue factor expression in vascular smooth muscle cells.

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posted on 2014-10-24, 15:29 authored by Maimaiti Yisireyili, Shinichi Saito, Shaniya Abudureyimu, Yelixiati Adelibieke, Hwee-Yeong Ng, Fuyuhiko Nishijima, Kyosuke Takeshita, Toyoaki Murohara, Toshimitsu Niwa

Serum-starved HASMCs (5×103 cells/well) in a 24-well plate were stimulated with or without IS (250 µmol/L) or prorenin (20 nmol/L) for 24 h (A, B). HASMCs were transfected with siPRR (20 nmol/L) for 48 h, before stimulation with IS or prorenin for 24 h. Thereafter, the cell proliferation reagent MTS (50 µL) was added to each well, and cells were incubated for 4 h. The absorbance was measured at 492 nm using a microplate reader (mean±SE, n = 3). **p<0.01 vs untreated group, #p<0.01 vs IS-treated group. HASMCs were transfected with or without PRR siRNA (20 nmol/L), and then serum starved for 24 h, followed by incubation with IS (250 µmol/L) or prorenin (20 nmol/L) for 24 h. Cell lysates were immunoblotted using anti-PRR and anti-tissue factor antibodies (C–F). Mean±SE (n = 3). *p<0.05, **p<0.01 vs untreated group, #p<0.05 vs IS-treated group. Ctrl: control.