IRF3 phosphorylation is essential for PISA and T. gondii replication.
Parasite infection and growth analyses were performed as described in Fig. 1A. Immunoblotting detected the indicated proteins, including phospho-IRF3 (P-IRF3), using a phospho-Ser396-specific antibody. A, The cell lines used are indicated. B, IRF3 KD (knocked-down) HT1080 cell line or these cells, reconstituted with WT or 396/98AA mutant of IRF3, were used for T. gondii infection. C, The indicated TBK1 KO and FLAG-TBK1-restored MEFs were used for T. gondii infection. Where shown, parasite growth was quantified by qPCR of genomic DNA.