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IRF3 phosphorylation is essential for PISA and T. gondii replication.

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posted on 2015-03-26, 03:30 authored by Tanmay Majumdar, Saurabh Chattopadhyay, Evgeny Ozhegov, Jayeeta Dhar, Ramansu Goswami, Ganes C. Sen, Sailen Barik

Parasite infection and growth analyses were performed as described in Fig. 1A. Immunoblotting detected the indicated proteins, including phospho-IRF3 (P-IRF3), using a phospho-Ser396-specific antibody. A, The cell lines used are indicated. B, IRF3 KD (knocked-down) HT1080 cell line or these cells, reconstituted with WT or 396/98AA mutant of IRF3, were used for T. gondii infection. C, The indicated TBK1 KO and FLAG-TBK1-restored MEFs were used for T. gondii infection. Where shown, parasite growth was quantified by qPCR of genomic DNA.