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IL-33 induces the expression of pro-inflammatory cytokines in HTNV-infected HUVECs.

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posted on 2015-02-06, 06:00 authored by Yusi Zhang, Chunmei Zhang, Ran Zhuang, Ying Ma, Yun Zhang, Jing Yi, Angang Yang, Boquan Jin

HUVECs were infected with HTNV/mock virus (MOI = 1) for 48 h or stimulated with IL-33 (20 ng/ml) for 6 h or first infected with virus for 48 h and then treated with IL-33 (20 ng/ml) for another 6 h. The mRNA expression of IL-1β, IL-6, IL-8, CCL2, CCL20, CXCL1, CXCL2, and CX3CL1 was determined by real-time PCR. Untreated HUVECs were set as the normal control (NC). Data are shown as the mean ± SD of triplicate samples and are representative of experiments with three independent HUVEC donors, *p < 0.05, **p < 0.01, ***p < 0.001.