IL-15 induced activation of HCMV and EBV-specific CD8 T cells.
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A) PBMC of 4 healthy volunteers were incubated with IL-15 (10 ng/ml), IL-2 (20 U/ml), IL-7 (10 ng/ml), IFN-γ (100 U/ml), IFN- α (100 U/ml), TNF- α (100 U/ml). Cells were harvested at the indicated time points and frequency of CD38/HLA-DR expressing virus specific CD8 T cells was analyzed. B) Contour plots show the CD38/HLA-DR expression of EBV, HCMV or Influenza specific CD8 T cells after 48 hours of incubation with the indicated cytokines in a representative healthy subject.