IL-10 DC generated in the presence of IFN-α induced an enhanced T cell activation.
(A, B) CD4+ or CD8+ T cells were stimulated in primary culture with allogeneic mDC, IL-10 DC or IL-10 DC cultured with 104 U/mL IFN-α. (A) T cell proliferation after restimulation was assessed as described and pooled data of 6 (CD4+ T cells) or 3 (CD8+ T cells) independent experiments are demonstrated. Proliferation is demonstrated in % +/−SD (normalized to 100% proliferation induced by control mDC). (B) IFN-γ and IL-10 production were detected in the supernatants of CD4+ T cells at day 5 of primary culture by ELISA. Mean values of cytokine levels in pg/ml +/− SD of 6 (IFN-γ) or 5 (IL-10) independent experiments are shown. * p<0.05; ** p<0.01; n.s. not significant.