IFT74 co-localizes with rootlet microtubules.

Immunofluorescent analysis showed IFT74 co-localized with rootlet microtubules in wild type, fla10 null and ift88 cells. IFT74 and IFT139 also were found in dots throughout the cytoplasm. The staining of acetylated tubulin (Ac-Tubulin) marked the rootlet microtubules and basal bodies. The arrows point to the sites co-stained with both IFT74 and acetylated tubulin antibodies. Scale bar, 10 µm.