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IFN-I signaling on hematopoietic cell lineage helps protect mice from lethal influenza infection.

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posted on 2011-02-24, 02:03 authored by Sang-Uk Seo, Hyung-Joon Kwon, Hyun-Jeong Ko, Young-Ho Byun, Baik Lin Seong, Satoshi Uematsu, Shizuo Akira, Mi-Na Kweon

Bone marrow (BM) cells from naïve WT (W) or Ifnar1−/− (K) mice were grafted to lethally irradiated WT or Ifnar1−/− recipient mice. (A–C) Sets of BM grafted mice were challenged with influenza PR8 virus (1×105 pfu) and analyzed at 3 dpi. (A) Number of monocytes (Mo) and neutrophils (N) were determined. (B) Expression levels of MCP-1 and KC were measured in BALF. (C) Monocytes in the lung were pooled and cultured for 4 h to measure chemokine production in vitro. Values are mean + SD of triplicate experiments with n>4 for each group. (D) Mice were challenged with lethal dose of influenza PR8 (1×105 pfu) and survival was monitored for 2 weeks. W→W (n = 17), K→W (n = 16), W→K (n = 16), and K→K (n = 11). Data were pooled from three independent experiments.