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IFC and cell sorting pictures.

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posted on 23.11.2015, 14:48 by Rosa I. Figueroa, Carlos Dapena, Isabel Bravo, Angeles Cuadrado

IFC images show cell and nuclear morphologies in cells with different DNA contents as seen using bright field microscopy and after blue (488 nm, nuclei in orange) and violet (405 nm, nuclei in red) laser excitation respectively. To the right of IFC images are shown sorted cells with the same DNA content as the cells shown by the IFC pictures (A, B, D + subscript). All cells have the nuclei stained with IP. 2-clusters were additionally stained with calcofluor (in blue) in sorted cells.