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Hypoxia increases the expressions of Oct3/4 and Nanog.

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posted on 2013-02-20, 10:28 authored by Yuanyuan Ma, Dongming Liang, Jian Liu, Karol Axcrona, Gunnar Kvalheim, Trond Stokke, Jahn M. Nesland, Zhenhe Suo

(A) In comparison to the cells cultivated at 20% O2, the cells cultivated at 7% O2 show higher levels of Oct3/4 and Nanog expressions, and the cells cultivated at 1% O2 show the highest levels of Oct3/4 and Nanog expressions in PC-3 and DU145 cell lines by both RT-PCR and immunoblotting. (B) Immunocytochemistry reveals corresponding higher levels of Oct3/4 and Nanog expressions at 7% O2 and the highest levels of Oct3/4 and Nanog expressions at 1% O2, in comparison to the cells cultivated at 20% O2 for both cell lines. Human seminoma tissue sections were used as positive controls for these two antibodies. All photos were originally taken at 200× and all the insets were taken at 400×.

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