Hyperthermia enhances HIV replication.
A: Experimental Outline. Jurkat or primary CD4+ T cells were infected with HIV-1 NL4-3 for 2 hours at 37°C and grown at either 37°C or 39.5°C for up to 10 days. B, D. Representative experiments with Jurkat and primary CD4+ T cells, respectively. The levels of intracellular Gag were measured by flow cytometry at the indicated time points. C, E: Mean ± SD of 5 independent experiments in Jurkat and primary CD4+ T cells, respectively. The area under the curve was calculated for all time points in Jurkat cells, and for time points occurring until the appearance of the peak at 39.5°C in primary CD4+ T cells. Statistical significance was assessed by the Wilcoxon test. p<0.05(*).