Hyper-oxaluric rats showed crystal deposits of Calcium Oxalate in renal tubules after 3 weeks.
(a) Hematoxylin and Eosin stained paraffin embedded 5 um sections were photographed under polarized light to detect crystal deposits in the tubules. Representative Images captured using a 10× objective magnification are shown. (b) Von Kossa's staining method was used to identify Calcium deposition in tubules of hyper-oxaluric rat kidneys. Digital photo-micrographs were taken with a 10× magnification. Representative images are shown.