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Histone acetylation occurs directly at the promoter regions of CXCL8 and CXCR1/2.

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posted on 2013-02-20, 20:57 authored by Anne-Marie Baird, Steven G. Gray, Kenneth J. O'Byrne

The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 16 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetylated histone H3 (Ac H3) and H4 (Ac H4), histone H3 acetylated at lysine 9 and 14 (H3K9/K14Ac), histone H3 acetylated at lysine 9 (H3K9Ac), histone H3 acetylated at lysine 9 and phosphorylated at serine 10 (H3K9pS10), Histone H3 dimetylation marker at lysine 9 (H3K9Me2), dimetylation marker at lysine 4 (H3K4Me2) and methylation marker at lysine 4 (H3K4Me). The chromatin status at the promoter region of (A) CXCL8, (B) CXCR1 and (C) CXCR2 is shown. Input DNA serves as a positive control recommended by the manufacturer (Diagenode). A no antibody control was included to test for non specific carriage of DNA with histones. (M – DNA size ladder).

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