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Histological immunolocalisation of SPDS aminopropyltransferases in Arabidopsis.

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posted on 08.10.2012, 00:46 authored by Borja Belda-Palazón, Leticia Ruiz, Esmeralda Martí, Susana Tárraga, Antonio F. Tiburcio, Francisco Culiáñez, Rosa Farràs, Pedro Carrasco, Alejandro Ferrando

Paraffin-embedded sections of different organs of A. thaliana were incubated with anti-SPDS antibodies and an avidin-biotin-peroxidase detection system. Dark brown staining indicates SPDS antibody-specific reaction. Either transverse or oblique sections are shown for: (a) wild-type developed flower, (b) embryo sac, (c) stamen, (d) sepal, (e) wild-type flower primordia, (f) receptacle, (g) gynoecium (i) mature silique, (j, k) embryo, (m, n, o) leaf, (q, r, s) stem, and (u, v, w) root. Specificity of the signal is shown by using preimmune serum as control reactions in (h, l, p, t, x). Abbreviations: C, cotyledon; Ca, cambium; Ch, chloroplast; Co, cortex; E, epidermis; Em, embryo sac; En, peripheral endosperm; G, gynoecium; I, inner integument; N, nucleus; P, pith; Pa, palisade mesophyll; Pe, petal; Ph, phloem; R, radicle; S, embryo; Sc, seed coat; Se, sepal; Si, silique; Sp, stigmatic papillae; St, stamen; Sy, style; Ue, upper epidermis; V, vascular bundle; X, xylem. Bar = 100 µm in (a, e, i, m, q, u); 25 µm in (j, n, r, v); 10 µm in (b, c, d, f, g, h, k, l, o, p, s, t, w, x).