High throughput application of cryopreserved mouse sperm.
(a). Genetically modified (GM) male mice were grouped based on their predominant background (first column), and the number of unique GM lines within each of the backgrounds is shown (second column). In vitro fertilization was preformed employing either our new sperm cryopreservation and recovery methods (Frozen) or freshly collected sperm (Fresh). The number of females and oocytes utilized are shown in the fourth and fifth columns. Differences between the Frozen and Fresh sperm treatments were assessed using ANOVA and preplanned contrasts. Asterisks within a bar indicate a significant difference between the treatments within a background. (b). A subset of the previously created embryos using cryopreserved sperm was transferred directly to pseudopregnant recipients. Those embryos created using freshly collected sperm were cryopreserved prior to transfer, as shown in column four. Differences between the two treatments were assessed using ANOVA and preplanned contrasts. Asterisks within a bar indicate a significant difference between the treatments within a background. No p-value is reported in those instances where the sample size was too small for statistical analysis.