High resolution mapping of the Hpt mutation.
Fine mapping of the Hpt mutation by segregation analysis of the HPT/LeJ-Hpt/+ x CAST/EiJ) F1 hybrid x C3HeB/FeJ cross. All of the 182 N2 offspring typed for the Chromosome 4 markers had one allele derived from C3HeB/FeJ (A) and the other allele derived from either C57BL/6J (B) or CAST/EiJ (C). Shown are the AB (gray) and AC (white) genotypes of 25 markers in the six most informative mutant N2 mice (Hpt/+, genotype designation AB) recombinant between D4Mit176 and D4Mit202. The markers D4Mit 352, hptssr22, hptssr23, and hptssr25 are non-recombinant with the Hpt mutation in all six mice, and the new flanking markers D4Mit 199 and hptssr50 refine the candidate gene interval to a 6.7 Mb region.