Het GIP Tg mice exhibit reduced weight gain and insulin resistance on HF diet.
Het GIP Tg (Tg/+) and non-transgenic WT littermates (+/+, 5 weeks old) were placed on either a low fat (LF) or high fat (HF) diet, and 25 mM ZnSO4 was added to the drinking water of both Het GIP Tg and WT mice (n = 6/group). A. Body weight gain in Het GIP Tg and WT mice placed on LF or HF diet. B. Percentage fat gain in WT and Het GIP Tg mice. C. Percentage fat of WT and Het GIP Tg mice on 14 weeks old. D and F. Glucose levels during an insulin tolerance test in LF (D)- and HF (F) diet-fed Het GIP Tg and WT littermates. After 8 weeks of feeding, WT and Het GIP Tg mice (13 weeks old, n = 6/group) were fasted for 4 hours and subsequently injected with 0.75 U/kg insulin. Blood glucose levels measured at 0, 15, 30, 60 and 120 min following insulin administration. E and G. Quantification of AUC for the total glycemic excursions in D and F. All data represent the mean ± S.E.M. and significance was tested using ANOVA with a Newman-Keuls post hoc test or Student’s t test, where ** represents p<0.05 vs indicated group or WT littermates.