H. pylori-induced histone H3 Ser10 dephosphorylation is cagPAI-dependent in MKN45 cells.

MKN45 cells (1×10⁶) were treated with medium alone, wild-type H. pylori 26695 (HP 26695), or an isogenic cag deletion strain (8-1) (HP 8-1) in RPMI-1640 medium plus 5% FBS at MOI of 100:1 for various periods of time. The cells were washed, lysed and proteins were separated on a 15% SDS-polyacrylamide gel and transferred to nitrocellulose membrane, which was probed with rabbit anti-phospho-histone H3 Ser10 antibodies (p-H3S10). The original membrane was then stripped and re-probed with anti-total histone H3 antibodies to monitor protein loading. Blots are representative of three separate experiments with similar results (Panel A). Data are mean±SEM from three densitometry scans, adjusted with total histone H3, and expressed as fold changes over the appropriate control, *P<0.01 when compared with controls (Panel B).