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H. pylori accumulation near gastric surface after microscopic damage.

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posted on 17.07.2014, 02:52 authored by Eitaro Aihara, Chet Closson, Andrea L. Matthis, Michael A. Schumacher, Amy C. Engevik, Yana Zavros, Karen M. Ottemann, Marshall H. Montrose

Fluorescently labeled H. pylori or beads were added to luminal fluid bathing the exposed gastric mucosa. Two-photon damage was imposed on a microscopic region of the epithelium as described in Methods. Images show representative time course of confocal imaging of beads or H. pylori (green) in parallel with confocal reflectance (red), without (A) or with (B) photodamage (damage site indicated by asterisk) imposed directly after time zero. As indicated, each time series shows outcomes tracking labeled beads, H. pylori SSI (wild-type), ΔmotB, or ΔcheY. Bar = 50 µm.

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