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HS results in an increase in SIRT1 recruitment that is specific to the HSE within the hsp70 promoter.

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posted on 19.02.2013 by Rachel Raynes, Kathleen M. Pombier, Kevin Nguyen, Jessica Brunquell, Jamie E. Mendez, Sandy D. Westerheide

(A) Schematic representation of primers designed to amplify the HSE site and a nonspecific (NS) site at the hsp70 promoter. (B) SIRT1 is recruited to the hsp70 promoter HSE site during HS. HEK293 cells were exposed to a 42°C HS from 0 to 6 hours prior to chromatin immunoprecipitation (ChIP) with a SIRT1 antibody. SIRT1 is not recruited upon HS to a non-specific (NS) upstream hsp70 promoter site (C) or to the gapdh promoter (D). Results in B-D are in technical triplicates and are representative of biological duplicates. Statistical significance was measured by Student’s t test as compared to - HS (*P<0.05; ** P<0.01; ***P<0.001).

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