HS results in an increase in SIRT1 recruitment that is specific to the HSE within the hsp70 promoter.
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(A) Schematic representation of primers designed to amplify the HSE site and a nonspecific (NS) site at the hsp70 promoter. (B) SIRT1 is recruited to the hsp70 promoter HSE site during HS. HEK293 cells were exposed to a 42°C HS from 0 to 6 hours prior to chromatin immunoprecipitation (ChIP) with a SIRT1 antibody. SIRT1 is not recruited upon HS to a non-specific (NS) upstream hsp70 promoter site (C) or to the gapdh promoter (D). Results in B-D are in technical triplicates and are representative of biological duplicates. Statistical significance was measured by Student’s t test as compared to - HS (*P<0.05; ** P<0.01; ***P<0.001).