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HIV Gag-GFP VLP budding from yeast spheroplasts is not diminished by loss-of-function mutations in Rsp5.

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posted on 19.02.2013, 18:12 by Andrew P. Norgan, Jacqueline R. E. Lee, Andrea J. Oestreich, Johanna A. Payne, Eugene W. Krueger, David J. Katzmann

was transformed into an background complemented with , , or . (A) Localization of by fluorescence microscopy on the indicated live cells. (B) VLP budding assays were conducted with cells of the indicated genetic backgrounds, and whole cell extracts (W.C.) and VLP fractions were visualized by anti-GFP immunoblotting.