HD-PTP does not harbor lipid phosphatase activity.
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HD-PTP phosphatase activity toward synthetic phosphatidylinositol phosphate substrates was evaluated using a malachite green-based assay. The catalytic domain (CD) of HD-PTP wild type (WT), C1392S (C/S) or S1394A (S/A) were incubated with PI(3,4,5)P (A), PI(3,4)P (B), PI(3,5)P (C) and PI(3)P (D) and phosphate release was quantified as described in Materials and Methods. PTEN wild type (WT) and C132S (C/S) purified proteins were used as controls. Results represent the average of three independent experiments (+/−SD).