HCMV infection disrupts the actin microfilament network within the tissue.
(A) Tissues were infected at an MOI of 10 and morphological changes of cells were observed by GFP fluorescence and brightfield microscopy of live cells at 24 and 48 hpi. (B) Model tissues were mock treated, infected using ADgfp or UV-treated ADgfp and fixed at 24 hpi. Changes in the actin microfilament network were assayed by fluorescence labeling. Actin was detected using phalloidin (red), infected cells were identified by GFP expression, and nuclei by DAPI (blue). Tissue orientation is indicated by the double arrow.