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H3.3, H3.1, and H3K4me0 dynamics in oogenesis and spermatogenesis.

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posted on 09.06.2011 by Jackelyn K. Arico, David J. Katz, Johan van der Vlag, William G. Kelly

(A–H) Comparison of H3.3::GFP (A, Bi–Di) to H3K4me2 (Bii–Dii), and H3.1 (E, Fi–Hi) to H3K4me2 (Fii–Hii), in hermaphrodite germ cells. (A) H3.3 is low in the distal nuclei of the gonad (d), and accumulates as nuclei progress towards the proximal (p) end. H3.3::GFP is absent from X in pachytene nuclei (Bi; arrow), identified by lack of H3K4me2 (Bii). H3.3::GFP is present on all chromosomes in mature oocytes (Ci), as is H3K4me2 (Cii). H3.3::GFP is absent from X's in larval hermaphrodite (spermatogenic) pachytene nuclei (Di; arrow), coincident with the absence of H3K4me2 (Dii). (E) H3.1 is high in the distal region of the gonad (d), and is depleted as nuclei progress towards the proximal (p) end. H3.1 is enriched on the X in pachytene nuclei (Fi; arrow) as identified by lack of H3K4me2 (Fii). H3.1 is low on all chromosomes in mature oocytes (Gi), while H3K4me2 is abundant (Gii). H3.1 is enriched on the paired X's in larval spermatogenic pachytene nuclei (Hi; arrows) as identified by the absence of H3K4me2 (Hii). (I, Ji–Li) X chromosome enrichment for H3K4me0 in male germ cells. (I) In both male and hermaphrodite (not shown) germ cells, H3K4me0 is high in the distal region of the gonad (d) and is depleted as nuclei progress towards the proximal (p) end. H3K4me0 is enriched on the X in pachytene hermaphrodite nuclei (Ji; arrow), as identified by lack of H3K4me2 (Jii). H3K4me0 is low on all chromosomes in mature oocytes (Ki) while H3K4me2 is abundant (Kii). H3K4me0 is enriched on the X's in larval hermaphrodite (spermatogenic) pachytene nuclei (Li; arrow) as identified by lack of H3K4me2 (Lii). Antibodies as indicated (green) with DAPI counterstain (red). Scale bars, 5 um.

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