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H3.1, H3.3, and H3K4me0 dynamics in the early embryo.

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posted on 09.06.2011, 01:58 authored by Jackelyn K. Arico, David J. Katz, Johan van der Vlag, William G. Kelly

In all image sets, a merged image of H3K4me2 (green) and DAPI (red) are shown, while the adjacent gray scale image shows a separate channel corresponding to co-staining with antibodies against H3.1, H3.3, or H3K4me0 as indicated. (Ai) In a one-cell embryo, all chromosomes except the Xp (arrow) incorporate H3K4me2 (green), but very little H3.1 is present on any chromosome (Aii). In 2 cell embryos low levels of H3.1 are detected on all chromosomes, including the Xp (Bi; arrow). Maternally provided H3.3:GFP is immediately incorporated into all sperm chromosomes, including the Xp, in the one cell stage (Cii; arrow), and thereafter (Dii nuclei from two cell embryo). Despite the incorporation of maternal H3.3, the autosomes retain H3K4me2 and the Xp (Ei; arrow) still lacks this modification, and is enriched for H3K4me0 relative to the autosomes (arrow; Eii). By the 2 cell stage and thereafter (Fii and not shown) all chromosomes (including the Xp; arrow), have incorporated high levels of H3K4me0 (Fii), but the Xp still excludes H3K4me2 (Fi; arrow). Scale bars, 5 um.

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