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H2A.Z peaks inversely correlate with expression level and Nap/Hrp occupancy.

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posted on 2009-11-13, 01:50 authored by Luke Buchanan, Mickaël Durand-Dubief, Assen Roguev, Cagri Sakalar, Brian Wilhelm, Annelie Strålfors, Anna Shevchenko, Rein Aasland, Andrej Shevchenko, Karl Ekwall, A. Francis Stewart

(A–D) All data from the 20bp tiling arrays were ordered with respect to the initiating methionine of each gene, which is shown as zero and the dotted line. The data were then binned into five groups according to the expression level of the gene [52] as indicated in the box at the right. (A) H2A.Z-myc ChIP from WT. (B) H2A.Z ChIP from msc1Δ. (C) H3 ChIP from WT. (D) H2A.Z-myc ChIP from swr1Δ. E) IGRs enriched in H2A.Z inversely correlate with mRNA expression level except for the least expressed 5%. H2A.Z enrichment (log2) was plotted against absolute mRNA level (log2) in WT using a 150-gene moving average based on expression data from Wiren et al, 2005 [52]. F) Moving average plots of H2A.Z enrichment against Hrp1, Hrp3 and Nap1 binding at IGRs using ChIP data from Walfridsson et al, 2007 [51]. Genes were sorted according to increasing Hrp1, Hrp3, or Nap1 IGR enrichment, and moving average plots of Log2 H2A.Z IGR values were generated using a 150-gene moving average. G) Venn diagram that represents the overlap of IGRs either enriched (>1.5) or depleted (,0.6) in H2A.Z, with IGRs enriched in either Hrp3 or Nap1 (.1.8). P values were generated using the hypergeometric method, and represent the probability of producing the given overlap between each pair of the shown datasets, except for the two values related to WT H2A.Z >1.5×, which refer to the lack of overlap.