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Growth of siRNA transfected RD cells in glucose and galactose medium.

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posted on 14.06.2012 by Sylvie Callegari, Philip A. Gregory, Matthew J. Sykes, Jennifer Bellon, Stuart Andrews, Ross A. McKinnon, Miguel A. de Barros Lopes

At 24 hours post-transfection, cells were seeded into wells of a 96-well plate in DMEM medium containing 10% fetal bovine serum and either 4.5 g/L glucose (left panel) or 4.5 g/L galactose (right panel). Cell proliferation was determined daily using a luminescent cell viability assay. An untransfected cell line and the rho0 cell line were included as controls. Cell proliferation is shown as a percentage of the maximum cell growth (100%) of the untransfected control. Error bars represent the mean ± SEM for three independent measurements.