Growth inhibition of Drosophila Kc cells by F14512 and characteristic cytotoxic effects.
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(A) Antiproliferative properties of F14512 and reference Topo II poisons, measured through viable cell counting (Vi-CELL) in exponentially growing Drosophila Kc cells. Drugs were used at the following concentrations: F14512 1 µM (-□-) and 50 µM (-▪-); etoposide (VP16) 1 µM (-Δ-) and 50 µM (-▴-); and TOP-53 1 µM (-○-) and 50 µM (-•-); control cells (-◊-) were treated with 0.1% DMSO alone. Viability was measured after the treatment times shown. Mean values +SD of three independent experiments are reported. (B) Phase-contrast micrographs of Kc cells treated as shown with etoposide (VP16), TOP-53 and F14512 and photographed after 24 hours. Arrowheads point to aggregates of apoptotic debris (etoposide VP16, TOP-53) or to elongated and/or multinucleated cells (F14512). Bar: 5 µm. Note that F14512 treatment yields no evident sign of apoptosis at the concentrations used here. (C) Phase-contrast or fluorescence micrographs of intact Kc cells and DAPI-stained fixed cells, respectively, after 24 hours of treatment with etoposide (VP16) or F14512 at the concentrations shown. The black arrowhead in the etoposide-treated photograph indicates apoptotic bodies, DAPI-stained samples show signs of hypercondensed chromatin. Low concentrations of F14512 (1 or 5 µM) again yield large adherent, elongated and multinucleated cells, DAPI staining shows evidence of internuclear chromatin bridges (white arrowheads) Bar: 5 µm.