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Gross appearance and dimension of neuroblastoma cell-mesenchymal stromal cell (NBC-MSC) co-encapsulated collagen microspheres.

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posted on 17.12.2015, 08:09 by Pan Yeung, Hoi Shun Sin, Shing Chan, Godfrey Chi Fung Chan, Barbara Pui Chan

Microspheres with different percentage of NBCs and hence NBC:MSC ratios: (A): 0% NBC (100% MSC); (B): 20% NBC (80%MSC); (C): 50% NBC (50% MSC); (D): 80% NBC (20% MSC) and (E): 100% NBC (0% MSC) were cultured for different period of time: 0 (A1,B1,C1,D1,E1); 1 (A2,B2,C2,D2,E2); 3 (A3,B3,C3,D3,E3), 7 (A4,B4,C4,D4,E4), 14 (A5,B5,C5,D5,E5) and 21 (A6,B6,C6,D6,E6) days (scale bars: 100 μm: A2,A3,A4,A5,A6,B2,B3,B4,B5,B6,C2,C3,C4,C5,D2,D3,D4; 500 μm: A1,D5,E3,E4,E6; 1000 μm: B1,C1,C6,D1,D6,E1,E2,E5); (F): Line chart showing the temporal change in the dimension (mean±1SD) of the NBC-MSC-collagen microsphere populations during cultures.