Figure_3.tif (230.27 kB)
Download file

Gr1int CD11b+ FSClow cells predominate in the lungs of NOS2-/- mice.

Download (0 kB)
posted on 01.11.2013, 03:14 by Andrés Obregón-Henao, Marcela Henao-Tamayo, Ian M. Orme, Diane J. Ordway

Further analysis of CD11b+FSClow and CD11b+FSChigh cells was performed by staining with anti-Gr1 antibody. As determined by mean fluorescence intensity, CD11b+FSChigh had significantly higher Gr1 expression than CD11b+FSClow (A). Starting at early time points NOS2-/- mice but not WT C57BL/6 mice have a significant number of cells giving an intermediate staining pattern for Gr-1 (B). At later time points, the influx of CD11b+FSChigh cells into the lungs of NOS2 -/- mice is also enhanced (C). Results are expressed as the Log mean cell number (± SEM, n=5) in the lung. Gr1high CD11b+ FSChigh cells express markers compatible with a monocytic lineage. Both populations of Gr1 expressing cells were further analyzed by flow cytometry using the monocytic markers Ly6C, CD14 and F4/80 (D). As determined by the mean fluorescence intensity for these markers, a more precise phenotype for these two cellular populations would be Gr1high CD11b+ FSChigh Ly6Chigh CD14+ F4/80+ and Gr1int CD11b+ FSClow Ly6Clow CD14low F4/80low, compatible with a monocytic and granulocytic lineage, respectively. Results are expressed as the log mean cell number (± SEM, n=5) in the Lung. ***Student t test, * p<0.05, ** p<0.01, *** p<0.001.


Usage metrics