Glomerular endotheliosis and injury in CD73^−/−mice.

Electrone microscopic analysis was performed n = 7 CD73^−/−mice vs. WT, original magnification x 10000 or 5000. (A) Ultrastructural analysis of the glomerular filtration barrier from a 3 months old WT mouse with regular appearance of foot processes and slit membranes and fenestration of the endothelium. (B) Analysis of the glomerular filtration barrier from a 3 months old CD73^−/− mouse shows cytoplasmic swelling apparent in endothelial cells (cap. Lumen  =  capillary lumen, ery  =  erythrocyte), endothelial fenestrations and single foot effacements were reduced (arrow). No subepithelial depositis were detectable. (C) Overview of a glomerular segment with regular appearance and without glomerulitis (3 months old WT-mouse). (D) Overview of a glomerular segment in a 3 months old CD73^−/−mouse (mes  =  mesangial cell, ly  =  lymphocytes) shows lymphocytic glomerulitis.