Global retrotransposon transcript levels and copy numbers increase as a result of decreased dCAP-D3 expression in somatic cells.
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A) qRT-PCR for 6 different retrotransposon transcripts in SG4 cells demonstrates that after 4 days of dCAP-D3 dsRNA treatment (white bars), there is a slight increase in global transcript levels as compared to cells treated with control dsRNA (black bars). B) qRT-PCR performed on cDNA from 20 wild type (w1118-black bar) or 20 dCap-D3 mutant (dCap-D3c07081dCap-D3/Δ25-white bar) larval brains indicates that a significant decrease in dCAP-D3 transcripts in vivo results in a slight increase in mdg1 transcripts. All qRT-PCR results were normalized to housekeeping gene rp49 and experiments are the average of three biological replicates. C) qPCR to determine relative global copy numbers of mdg1, G2, and X element in wild type and dCap-D3 mutant larvae indicates that copy numbers increase slightly in the mutants. Copy numbers for each retrotransposon were normalized to single copy regions located upstream. Analyses were performed on pooled samples of DNA from 10 larvae for each genotype. (*) indicates p-value less than 0.05 as calculated by student unpaired t-test.