Genomic fragments of type 2 VDPVs amplified by RT-PCR for sequencing and phylogenetic analysis.
The polyprotein encoded by the Sabin 2 genome is represented by a rectangle on the genomic RNA.
The 5′ UTR (partial), P1, VP1, and 3D polymerase regions that were amplified by RT-PCR and sequenced are represented by 4 rectangles with dark cross hatching below the genomic RNA.
They correspond to nt 183 to 747, 748 to 3384, 2482 to 3384, and 5986 to 7368, respectively, of Sabin 2 (Acc: X00595).
The downward pointing arrow indicates the start of the hypervariable region of 5′UTR.
The upward-pointing arrow indicates the location of the genetic recombination in the 3D polymerase gene.