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Genome-wide polyubiquitylation activities on meiotic chromosomes are reduced in UBR2−/− spermatocytes.

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posted on 2012-05-17, 02:16 authored by Jee Young An, Euna Kim, Adriana Zakrzewska, Young Dong Yoo, Jun Min Jang, Dong Hoon Han, Min Jae Lee, Jai Wha Seo, Yong Jun Lee, Tae-You Kim, Dirk G. de Rooij, Bo Yeon Kim, Yong Tae Kwon

Surface-spread meiotic chromosomes were coimmunostained with FK1 antibody (red) which specifically recognizes polyubiquitin conjugates and an antibody to SCP3 (green), a component of the synaptonemal complex. (A) Zygotene. (B) Early pachytene. (C) Mid-pachytene. Arrowhead indicates the sex chromosome. Note that polyubiquitin signals are virtually nondetectible in UBR2−/− chromosomes. Scale bar: 10 µm.