Genome-wide expression profiling.
Whole blood gene expression profiles (Panel A). Whole blood cell expression profiles before (0), 2 h and 24 h post CPB revealed 916 differentially regulated transcripts corresponding to 610 known genes (p<0.01). The selected sets of genes are presented as a heatmap using hierarchical clustering (HCL) with Euclidean distances and complete linkage analysis (for a detailed gene list see supplemental SOM table S1). Each row represents lg2 expression ratios of an individual gene (CS/CPB vs. average intensity of all measurements) and the columns indicate each respective patient (i.e., sample time points). Expression ratios are colored according to the scale bar: green = downregulation, red = upregulation. Representative CS/CPB-induced genes are highlighted in the red box. Selected CS/CPB-induced genes are highlighted in the red box. Functional processes (Panel B). The top 10 functional processes affected by CS/CPB treatment are presented. Data analysis among CS/CPB regulated genes from the selected gene set resulted in significant enrichment for gene ontology processes and cell survival or death. Bars represent -log p-values representing the probability for the gene ontology mapping arising by chance.